An enzyme substrate is a substance that is acted upon by an enzyme. Since many enzyme substrates are peptides, New England Peptide | Peptides International carries an abundance of enzyme substrates, including FRETS (Fluorescence Energy Transfer Substrates). Whether you are working in cancer research, evaluating inhibitors of SARS-CoV-2 for COVID-19 research, or looking to track an enzyme’s activity, a substrate may be just the tool you need.
A few of our popular substrate categories are highlighted below:
Chymotrypsin and Chymotrypsin-like Protease Substrates
Chymotrypsin is a serine protease and a digestive enzyme. Chymotrypsin is activated after proteolysis of chymotrypsinogen by trypsin. Chymotrypsin cleaves peptide bonds formed by aromatic residues (tyrosine, phenylalanine, and tryptophan).
Collagenase / MMP / Stromelysin Substrates
The Matrix Metalloproteinases (MMPs) are involved in the degradation of the extracellular matrix (ECM) and are thought to provide a pathway for invasion by cancerous tumor cells.
Fluorogenic Substrates for SARS-CoV/SARS-CoV-2 Main Protease (Mpro)
Trypsin and Trypsin-like Protease Substrates
Like chymotrypsin, trypsin is a serine protease and a digestive enzyme. Its main function is the hydrolysis of proteins. Trypsin cleaves a protein from the C-terminal side of a lysine or arginine.
We also carry and can custom synthesize tryptic peptides, or TrypTidesTM, here.
For the complete list of substrates, take a look at the category here, or search our online catalog. If you are looking for something we do not carry, or want to change the sequence or fluorophore, we can also customize a substrate. Request a quote today.
Here is an interesting publication that looks at connection between enzyme and substrate and the Michaelis-Menten equation: Intrinsically Disordered Linkers Control Tethered Kinases Via Effective Concentration