Glucose Uptake Monitoring in Living Cells, Reagent 2-NBDG

A single, living, & malignant cells fluorescent glucose derivative was first developed for measurement of glucose uptake activity in E. coli and for rapid determination of non-culture counts of bacteria.1 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2deoxy- D-glucose (2-NBDG), accumulates in living but not dead cells and allows for analysis of glucose uptake into cells in real time.

Discovery that 2-NBDG uptake into E.coli and C. albicans was blocked by d- but not l- O glucose, indicated the involvement of glucose HO transporters (GLUTS) for entry into cells. This HO OH was later confirmed in studies where 2-NBDG HN treatment of cells overexpressed with GLUT2 led to significant increase in fluorescence compared to mock-transfected cells.

 

Authors from Peptides International