Phospholipase A2 P00630 Bee Venom Protein Recombinant
- Product Code: ALR-001
- Synonyms/Product Alternate Names: Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Allergen Api m I, Api m 1.
Introduction: Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies.
Description: The E.Coli derived recombinant protein contains phospholipase P00630 bee venom protein epitopes, 26-162 amino acids. Phospholipase A2 P00630 Bee Venom is fused to a 6xHis tag at C-terminus
Purification Method: Purified by proprietary chromatographic technique.
Formulation: 20mM Tris-HCl, pH 7.2, 1.5M urea and 50% glycerol.
Stability: Phospholipase A2 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
- Origin: Escherichia coli
- Storage Conditions: -20 °C
- Purity: >90% pure as determined by 10% SDS-PAGE.
Delivery Details: All quantities are subject to availability. Please allow 7-10 days for delivery.