Phospholipase A2 P00630 Bee Venom Protein Recombinant

  • Product Code: ALR-001
  • Synonyms/Product Alternate Names: Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Allergen Api m I, Api m 1.
  • Introduction: Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies.

    Description: The E.Coli derived recombinant protein contains phospholipase P00630 bee venom protein epitopes, 26-162 amino acids. Phospholipase A2 P00630 Bee Venom is fused to a 6xHis tag at C-terminus

    Purification Method: Purified by proprietary chromatographic technique.

    Formulation: 20mM Tris-HCl, pH 7.2, 1.5M urea and 50% glycerol.

    Stability: Phospholipase A2 although stable at 4°C for 1 week, should be stored below -18°C.
    Please prevent freeze thaw cycles.

  • Origin: Escherichia coli
  • Storage Conditions: -20 °C
  • Purity: >90% pure as determined by 10% SDS-PAGE.

Delivery Details: All quantities are subject to availability. Please allow 7-10 days for delivery.

  • 100µg
  • $150.00
  • 500µg
  • $600.00
  • 1000µg
  • $1,000.00
For research use ONLY. Not for use on humans.