Orexin-A and Orexin-B Available from Peptides International

Neuropeptides Stimulate Food Consumption: Orexin-A and Orexin-B

Orexin Peptides Available from Peptides International

Orexin-A and Orexin-B peptides (Gk. orexis, appetite) were isolated from rat brain extracts in 1998.¹ These peptides were discovered in effort to identify the endogenous ligands for two closely related orphan G protein-coupled receptors, OX₁R and OX₂R. These neuropeptides stimulate food consumption upon central administration in rats. Orexin-A is a 33-residue peptide with two intramolecular disulfide bonds, while orexin-B is a linear 28-residue peptide. Orexin-B was found to be 46% (13/28) identical in sequence to orexin-A. The primary structure of orexin-A is completely conserved among human, rat, mouse and bovine species. When orexin-A was administered intracerebroventricularly, food intake was stimulated in a dose-dependent manner within 1 hour. Human orexin-B also significantly stimulated food intake yet the effects were not as long-lasting as those of orexin-A. prepro-orexin mRNA and orexin-A are localized in neurons within and around the lateral and posterior hypothalamus in adult rat brains. Furthermore, levels of prepro-orexin mRNA were found to be up-regulated upon fasting. The role of these peptides as mediators in the central feedback mechanism that regulates feeding behavior is under intense investigation. The discovery of these peptides may be valuable tools for such diseases involving disregulation of energy homeostasis such as, obesity and diabetes mellitus.

1. T. Sakurai, A. Amemiya, M. Ishii, I. Matsuzaki, R.M. Chemelli, H. Tanaka, S.C. Williams, J.A. Richardson, G.P. Kozlowski, S. Wilson, J.R.S. Arch, R.E. Buckingham, A.C. Haynes, S.A. Carr, R.S. Annan, D.E. McNulty, W.-S. Liu, J.A. Terrett, N.A. Elshourbagy, D.J. Bergsma and M. Yanagisawa, Cell, 92, 573 (1998).

CODE	PRODUCT	QTY	USD
POR-4346-s	Orexin-A (Human) (Rat, Mouse, Bovine) Pyr-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr- Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly- Ala-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Leu-NH₂ M.W. 3561.2 (Disulfide bonds between Cys⁶-Cys-¹² and Cys⁷-Cys¹⁴) (M.W. 3561.20 ) C₁₅₂H₂₄₃N₄₇O₄₄S₄ Appetite-Boosting Peptide	0.1 mg vial	215
POR-4348-s	Orexin-B (Human) Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu- Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala- Ala-Gly-Ile-Leu-Thr-Met-NH₂ (M.W. 2899.40 ) C₁₂₃H₂₁₂N₄₄O₃₅S Appetite-Boosting Peptide	0.1 mg vial	107
POR-4347-s	Orexin-B (Rat, Mouse) Arg-Pro-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu- Gln-Arg-Leu-Leu-Gln-Ala-Asn-Gly-Asn-His-Ala- Ala-Gly-Ile-Leu-Thr-Met-NH₂ (M.W. 2936.40 ) C₁₂₆H₂₁₅N₄₅O₃₄S Appetite-Boosting Peptide	0.1 mg vial	107
	Orexin References T. Sakurai, A. Amemiya, M. Ishii, I. Matsuzaki, R.M. Chemelli, H. Tanaka, S.C. Williams, J.A. Richardson, G.P. Kozlowski, S. Wilson, J.R.S. Arch, R.E. Buckingham, A.C. Haynes, S.A. Carr, R.S. Annan, D.E. McNulty, W.-S. Liu, J.A. Terrett, N.A. Elshourbagy, D.J. Bergsma, and M. Yanagisawa, Cell, 92, 573 (1998). (Original) L. de Lecea, T.S. Kilduff, C. Peyron, X.-B. Gao, P.E. Foye, P.E. Danielson, C. Fukuhara, E.L.F. Battenberg, V.T. Gautvik, F.S. Bartlett II, W.N. Frankel, A.N. van den Pol, F.E. Bloom, K.M. Gautvik, and J.G. Sutcliffe, Proc. Natl. Acad. Sci. USA, 95, 322 (1998). (cDNA; SAme Sequence [Hypocretin])

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