New Substrate for Collagenase 3/MMP-13 available from Peptides International

New Substrate for Collagenase 3/MMP-13

New substrate now available from Peptides International

Peptide substrates are useful for the determination of protease enzyme activity which is also an effective method for screening new potential therapeutic protease inhibitors. A new highly sensitive substrate for assaying MMP activities is now available. Evaluation requires HPLC separation of peptide fragments (monitored at l = 350 nm). The substrate cleavage between Gly-Met is determined by HPLC separation (monitored at l = 350 nm). k_cat/K_m (M^-1s^-1) at 37°C, pH 7.5, is 4.22 x 10⁶ for MMP-13, 3.9 x 10⁵ for MMP-9, 5.14 x 10³ for MMP-1, and 3.14 x 10³ for MMP-3. The specific substrate described below has been especially developed for the accurate determination of MMP-13 activity and will help users monitor the effectiveness and specificity of potential inhibitors.

S.J. Deng, D.M. Bickett, J.L. Mitchell, M.H. Lambert, R.K. Blackburn, H.L. Carter, III, J. Neugebauer, G. Pahel, M.P. Weiner, M.L. Moss, J. Biol. Chem., 275, 31422-31427 (2000).

CODE	PRODUCT DESCRIPTION	QTY	USD
SDP-3816-PI NEW!	Dnp-Gly-Pro-Leu-Gly-Met-Arg-Gly-Leu-NH₂ 2,4-Dinitrophenyl-Glycyl-L-Prolyl-L-Leucyl-Glycyl-L- Methionyl-L-Arginyl-Glycyl-Leucine Amide (M.W. 965.11) C₄₀H₆₄N₁₄O₁₂S Substrate for Collagenase 3/MMP-13 Purity: >95% by HPLC. Supplied as a trifluoroacetate salt.	1 mg 5 mg	45 175
Larger quantities available; please contact our technical sales specialists to discuss your project needs..
Please contact Peptides International for ordering information. Peptides International, Inc. PO Box 24658 Louisville, Kentucky 40224 USA Phone: 502-266-8787 or 800-777-4779 Fax: 502-267-1FAX (1329) Email: peptides@pepnet.com
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